Difference between revisions of "Part:BBa K2217013"

Revision as of 17:06, 23 October 2017

CAG Promoter

We tried to improve CAG promoter at BBa_K1179069 by making it RFC 10 compatible. CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2217013 short</partinfo>
-CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC is repeated 6 consecutive times starting at base 538. reduced below 6. Note that alternate codons may be used to reduce repeated regions in a coding sequence. A homopolymer run of 16 G bases is present starting at base 413. become less than 9.
+We tried to improve CAG promoter at [https://parts.igem.org/Part:BBa_K1179069# BBa_K1179069] by making it RFC 10 compatible.
+CMV enhancer + chicken beta-actin promoter, the part was improved by sequence adjustment to gene block synthesis requirements The sequence GGC was repeated 6 consecutive times starting at base 538 and a homopolymer run of 16 G bases is present starting at base 413 was reduced less than 9.
 <!-- Add more about the biology of this part here