Difference between revisions of "Part:BBa K2243003"
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<partinfo>BBa_K2243003 short</partinfo> | <partinfo>BBa_K2243003 short</partinfo> | ||
− | Through the random mutation from iGEM registry RBS, one well-behaved RBS | + | Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct. |
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Revision as of 16:13, 23 October 2017
D7_Bxb1 gp35
Through the random mutation from iGEM registry RBS, one well-behaved RBS D7 is constructed upstream Bxb1 gp35 coding sequence. The Bxb1 gp35 integrase performed high efficiency of DNA inversion using this construct.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 192
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 466
Illegal XhoI site found at 553 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1105
Illegal NgoMIV site found at 1192
Illegal AgeI site found at 242 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1300