Difference between revisions of "Part:BBa K418007:Experience"

Latest revision as of 15:43, 23 October 2017

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K418007

User Reviews

UNIQc5b28e3ee52e8790-partinfo-00000000-QINU UNIQc5b28e3ee52e8790-partinfo-00000001-QINU

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TU_Darmstadt

For our project, we also need an enzyme which produces chitin. We considered using this CHS3 from Northwestern, but after taking a closer look we decided otherwise. The CHS3 is a transmembrane protein originating from eukaryotes, which is challenging to integrate into prokaryotes, although the Northwestern team used the cDNA. Furthermore, the enzyme is post-translationally glycosylated, which makes it nearly impossible to express a functional enzyme.
We made a new part that will be working in prokaryotes Part:BBa_K2380000

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+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_K418007 AddReview 1</partinfo>
+<I>TU_Darmstadt</I>
+|width='60%' valign='top'|
+For our project, we also need an enzyme which produces chitin. We considered using this CHS3 from Northwestern, but after taking a closer look we decided otherwise. The CHS3 is a transmembrane protein originating from eukaryotes, which is challenging to integrate into prokaryotes, although the Northwestern team used the cDNA. Furthermore, the enzyme is post-translationally glycosylated, which makes it nearly impossible to express a functional enzyme.<br>
+We made a new part that will be working in prokaryotes [https://parts.igem.org/Part:BBa_K2380000| Part:BBa_K2380000]
+|}