Difference between revisions of "Part:BBa K2368005"
Line 4: Line 4:
 
<partinfo>BBa_K2368005 short</partinfo>
 
<partinfo>BBa_K2368005 short</partinfo>
  
−
<P><i>P<sub>fus</sub></i> is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in <i>S.cerevisiae</i>,</P> when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast.  
+
<P><i>P<sub>fus</sub></i> is an induced promoter from <i>Saccharomyces cerevisiae</i>. In the endogenous MAPK pathway in <i>S.cerevisiae</i>, when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. </P>
−
<P>However, the sequence of <i>P<sub>fus</sub></i> contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
+
<P>However, the sequence of <i>P<sub>fus</sub></i> contains <i>Pst1</i> restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
 
<div id="section">
 
<div id="section">
 
<div id="section">
 
<div id="section">

Revision as of 14:43, 23 October 2017


Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

标题 ‎

fig.1 The sequencing result of Pfus

This part is an improvement of BBa_K775004 of 2013 SCUT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]