Difference between revisions of "Part:BBa K2267001"

 
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<partinfo>BBa_K2267001 short</partinfo>
 
<partinfo>BBa_K2267001 short</partinfo>
  
GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.https://static.igem.org/mediawiki/parts/8/8d/GlsA%E6%8F%92%E5%9B%BE.docx
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GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.[[File:T--TUST China--parts_GlsA_pcr.png]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:41, 23 October 2017


A gene encodes glutaminase 1;Catalyze a reaction converting glutamine to glutamate and releasing the

GlsA encodes an acid-activated glutaminase, which is sufficient for an acid resistance system, and it is able to catalyze a reaction converting glutamate to glutamine and releasing the free gaseous ammonia. The free gaseous ammonia will consume the proton and increase the intracellular pH.T--TUST China--parts GlsA pcr.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
    Illegal NotI site found at 9
    Illegal NotI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 967
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 967
    Illegal SpeI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]