Difference between revisions of "Part:BBa K2350023"

 
 
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To ensure that our transform gene can successfully express on the PCC7942, we, therefore, ligate it with a special well-designed backbone which designed by ourselves and could express in PCC7942.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 12:34, 23 October 2017


pPIGBACK-PrbcL-CrtW-CrtZ-rrnB

To ensure that our transform gene can successfully express on the PCC7942, we, therefore, ligate it with a special well-designed backbone which designed by ourselves and could express in PCC7942.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1299
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1861
    Illegal BglII site found at 4285
    Illegal BglII site found at 5025
    Illegal BamHI site found at 2158
    Illegal BamHI site found at 5679
    Illegal XhoI site found at 1096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 950
    Illegal BsaI site found at 1761
    Illegal BsaI site found at 5881