Difference between revisions of "Part:BBa K2346000:Experience"
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| + | To assay the reactivity of the protein. We put the enzyme, sodium nitrite and pbs buffer together to establish a reaction system. We recorded the nitrite concentration over 24 hours, under temperature of 37 degree Celcius. The nitrite concentration was measured using a color-showing reagent consisting of p-aminobenzenesulfonamide and N-(1-Naphthyl)ethylenediamine dihydrochloride. The reagent reacts with nitrite to form the redish pink substance(diazonium salt) in the solution. The formed substance has a maximum absorbance at 540nm. A standard curve was first made by measuring the absorbance at 540nm of the reaction system under various nitrite concentration. | ||
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| + | According to our results, the absorbance dropped from 0.633 to 0.008 over 24 hours, which indicates a drop of nitrogen concentration from 0.196 to roughly zero. Currently we are still testing samples with shorter and various reaction time-length to plot the closest curve of the reactivity. | ||
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Latest revision as of 10:10, 23 October 2017
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