Difference between revisions of "Part:BBa K2384007:Design"

 
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===Design Notes===
 
===Design Notes===
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It is more suitable for expression in Bacillus megaterium.And there is no termination codon in this CDS.
+
It is more suitable for expression in Bacillus megaterium.And there is no termination codon in this CDS.And this CDS is with a T7 terminator.
  
  

Revision as of 07:27, 23 October 2017


GST-CRS5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

It is more suitable for expression in Bacillus megaterium.And there is no termination codon in this CDS.And this CDS is with a T7 terminator.


Source

Our sequence optimization is based on the sequence provided by Cornell University's IGEM VIKI in 2014.

References