Difference between revisions of "Part:BBa K2244004"

Revision as of 06:45, 23 October 2017

mheI

short description

This part is a functionalor MBC) hydrolyzing esterase that degrades MBC pesticide.

gene encoding a carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate,

long description

This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 591
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 250
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===short description===
-This part is a coding gene mheI (for MBC-hydrolyzing enzyme) that encodes MBC-hydrolyzing esterase (MHE) to degrade MBC pesticide. Degradation of MBC is achieved by hydrolyzing MBC to 2-aminobenzimidazole (2-AB), which is then converted to benzimidazole or 2-hydroxybenzimidazole (2-HB). The conversion of 2-AB was inhibited by NH4NO3. The benzene ring of 2-HB was further opened through meta catechol cleavage. MHE is responsible for carrying out the first step detoxification (MBC-2-AB), without the need of any cofactor. MHE is a soluble serine hydrolase of 242 amino acid residues and has a molecular size of 25-27 kDa.
+This part is a functionalor MBC) hydrolyzing esterase that degrades MBC pesticide.
+ gene encoding a carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate,