|
|
| Line 1: |
Line 1: |
| | | | |
| − | __NOTOC__
| |
| − | <h1>Introduction</h1>
| |
| − | <partinfo>BBa_K2368023 short</partinfo>
| |
| − |
| |
| − | <p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct T1R2-Myc tag. This tag can be recognized by specific monoclonal antibodies then we use fluorescence second antibody to interact with monoclonal antibodies to show the T1R2’s expression and location. </p>
| |
| − | <p>Because the T1R2 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein αsubunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R2 and Myc tag. And hope to show the location of T1R2 through immunofluorescence for detecting Myc tag.</p>
| |
| − |
| |
| − |
| |
| − | <!-- Add more about the biology of this part here
| |
| − | ===Usage and Biology===
| |
| − |
| |
| − | <!-- -->
| |
| − | <h2>Sequence and Features</h2>
| |
| − |
| |
| − | <partinfo>BBa_K2368023 SequenceAndFeatures</partinfo>
| |
| − |
| |
| − | <!-- Uncomment this to enable Functional Parameter display
| |
| − | ===Functional Parameters===
| |
| − |
| |
| − | <partinfo>BBa_K23680232 parameters</partinfo>
| |
| − | <!-- -->
| |
