Difference between revisions of "Part:BBa K2314212"
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UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short，it can be used as a combination of a promoter and a RBS. | UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short，it can be used as a combination of a promoter and a RBS. | ||
| − | + | <img src="https://static.igem.org/mediawiki/parts/2/25/T--OUC-China-utrrbs.png"/> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:31, 23 October 2017
UTRrpsT enhanced promoter
UTRrpsT by combining with J23108, to achieve the effect of enhancing J23108. This part contains J23108, UTRrpsT and RBS. UTRrpsT has the ability to enhance the promoter. If you need to enhance the expression of a promoter, you can add UTRrpsT way to achieve. The reason why UTRrpsT is not a separate part is the possibility that the prefix and suffix sequences form a special secondary structure on the 5'UTR. For more details you can view the papers.In short，it can be used as a combination of a promoter and a RBS.
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 56
- 1000COMPATIBLE WITH RFC[1000]