Difference between revisions of "Part:BBa K2332005:Design"

 
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===Source===
 
===Source===
  
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The reverse translation of EL222 (UniProtKB/Swiss-Prot: Q2NB98.1) optimized for E. coli was obtained as a synthetic gBlock along with the promoter, RBS and terminator sequences.
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Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
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===References===
 
===References===

Revision as of 00:19, 23 October 2017


EL222, photosensitive DNA-binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal AgeI site found at 367
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to reverse translate and codon optimize the protein sequence for its usage in E. coli and ensure no illegal sites were present.


Source

Sequence obtained from the reverse translation of EL222 (UniProtKB/Swiss-Prot: [http://www.uniprot.org/uniprot/Q2NB98 Q2NB98.1]) then codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References