Difference between revisions of "Part:BBa K2332042:Design"

(→‎Design Notes)
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===Source===
 
===Source===
  
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SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040
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SpyCatcher sequence was obtained from [https://parts.igem.org/Part:BBa_K1159200 BBa_K1159200] and GFP from:  
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[https://parts.igem.org/Part:BBa_E0040 BBa_E0040]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===

Revision as of 00:07, 23 October 2017


GFP-mutSpyCatcher (Lys31X, for photocaging)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


Design Notes

This construct was designed to not contain any other amber stop codon but at position 31 (Lys31X) (Amberless E. coli must be used to see the effects of photocageing spyCatcher). We removed the stop codon in GFP and placed the SpyCatcher sequence after a linker sequence and included a HisTag for protein purification.

Source

SpyCatcher sequence was obtained from BBa_K1159200 and GFP from: BBa_E0040. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References