Difference between revisions of "Part:BBa K2332014:Design"

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===Source===
 
===Source===
  
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All parts were obtained as synthetic gBlocks
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Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli.
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Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyCatcher: BBa_K1159200
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Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001.
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Pblind:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5001607/
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SpyCatcher was obtained from: [https://parts.igem.org/Part:BBa_K1159200 BBa_K1159200]. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
  
 
===References===
 
===References===

Revision as of 00:02, 23 October 2017


Blue light inducible expression of Intimin'-SpyCatcher (Pblind Intimin'-SpyCatcher)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1639
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to fuse SpyCatcher to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins (Wentzel et al., 2001). We also included a linker between Intimin and SpyCatcher to facilitate mobility of SpyCatcher on the cell surface and a HisTag for protein purification. Blue light inducible promoter was designed by Jayaraman P. et al. (2016)

Source

Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli. Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001. SpyCatcher was obtained from: BBa_K1159200. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References