Difference between revisions of "Part:BBa K2332011"

Revision as of 23:57, 22 October 2017

Blue light inducible expression of Intimin'-SpyTag (Pblind Intimin'-SpyTag)

This biobrick is the fusion of a truncated version of Intimin (159kDa) and SpyTag (13 amino acids) for the cell surface display of SpyTag under the control of our Pblind, blue light inducible promoter. To trigger cell adhesion, we decided to make use of the SpyTag and SpyCatcher, two binding partners that form rapid covalent bonds, fused to outer membrane surface proteins. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together but only post blue light induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2332011 short</partinfo>
-To trigger light inducible cell adhesion, we decided to use our blue light inducible promoter to trigger the synthesis of either SpyTag or SpyCatcher, two binding partners that form rapid covalent bonds. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together. This biobrick is the fusion of a truncated version of Intimin and SpyTag for the cell surface display of SpyTag under the control of a blue light inducible promoter.
+This biobrick is the fusion of a truncated version of Intimin (159kDa) and SpyTag (13 amino acids) for the cell surface display of SpyTag under the control of our [https://parts.igem.org/Part:BBa_K2332002 Pblind], blue light inducible promoter. To trigger cell adhesion, we decided to make use of the SpyTag and SpyCatcher, two binding partners that form rapid covalent bonds, fused to outer membrane surface proteins. SpyTag (13 amino acids) and SpyCatcher (138 amino acids, 15 kDa) come from CnaB2 (immunoglobulin-llike collagen adhesin domain) of the FbaB protein, found in the invasive strains of S. pyogenes. We decided to fuse SpyTag and SpyCatcher to outer membrane surface proteins in distinct cell populations that would then aggregate once mixed together but only post blue light induction.
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