Difference between revisions of "Part:BBa K2332001:Design"
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===References=== | ===References=== | ||
| + | Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005. | ||
Latest revision as of 23:50, 22 October 2017
Blue light inducible expression system with GFP reporter (Pblind GFP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 709
Design Notes
Pblind promoter was designed by Jayaraman P. et al. (2016)
Source
GFP sequence was obtained from: BBa_E0040 The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
References
Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.