Difference between revisions of "Part:BBa K2332053:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.
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We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.
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===Source===
 
===Source===

Revision as of 23:44, 22 October 2017


Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 723


Design Notes

We removed the stop codon in GFP and placed the SpyTag sequence after a linker sequence.

Source

https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence https://parts.igem.org/Part:BBa_E0040 was for the GFP

References