Difference between revisions of "Part:BBa K2332020:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: [https://www.ncbi.nlm.nih.gov/nuccore/5726577 AF170104.1], from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli. | ||
===Source=== | ===Source=== | ||
Revision as of 23:17, 22 October 2017
LuxCDABE (E. coli bioluminescence)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2562
Illegal AgeI site found at 2829
Illegal AgeI site found at 3357
Illegal AgeI site found at 3938
Illegal AgeI site found at 4385
Illegal AgeI site found at 5263
Illegal AgeI site found at 5478 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: AF170104.1, from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli.
Source
Vibrio fischeri regulatory protein LuxR (luxR) gene, complete cds; LuxICDABEG operon sequences were obtained from Genbank: AF170104.1, from which LuxC,D,A,B,E genes were identified and codon optimized for E. coli. The DNA sequence was synthesised by Integrated DNA Technologies (IDT)
References
Chong G, Kimyon Ö, Manefield M. Quorum Sensing Signal Synthesis May Represent a Selective Advantage Independent of Its Role in Regulation of Bioluminescence in Vibrio fischeri. PLoS ONE. 2013;8(6):e67443.