Difference between revisions of "Part:BBa K2332020:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The 5kb construct is too large to obtain as a single construct so it was split into gBlock fragments and each were designed to have 40bp overlaps to the adjacent fragment for gibson assembly.
 
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===Source===
 
===Source===

Revision as of 23:11, 22 October 2017


LuxCDABE (E. coli bioluminescence)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
    Illegal AgeI site found at 2829
    Illegal AgeI site found at 3357
    Illegal AgeI site found at 3938
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 5263
    Illegal AgeI site found at 5478
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Vibrio fischeri LuxR and Lux operon GenBank: AF170104.1 from which LuxC, D, A, B, E genes were identified and codon optimized for E. coli. The 5kb construct was split into 8 gblock fragments (700-900bp each) and ordered from IDT

References