Difference between revisions of "Part:BBa K2332020"

Latest revision as of 23:03, 22 October 2017

LuxCDABE (E. coli bioluminescence)

This part encodes the production of the proteins required for bacterial luminescence including luciferase and its substrates from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2562
Illegal AgeI site found at 2829
Illegal AgeI site found at 3357
Illegal AgeI site found at 3938
Illegal AgeI site found at 4385
Illegal AgeI site found at 5263
Illegal AgeI site found at 5478
1000
COMPATIBLE WITH RFC[1000]

Revision as of 23:00, 22 October 2017 (view source) Paola handal (Talk \| contribs) ← Older edit		Latest revision as of 23:03, 22 October 2017 (view source) Paola handal (Talk \| contribs)
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	<partinfo>BBa_K2332020 short</partinfo>		<partinfo>BBa_K2332020 short</partinfo>

−	This part encodes the production of the proteins required for bacterial luminescence including luciferase and its ~~subtrates~~ from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.	+	This part encodes the production of the proteins required for bacterial luminescence including luciferase and its substrates from Vibrio fischeri, codon optimised for E. coli. Naturally, Vibrio fischeri use additional regulatory proteins (encoded by LuxR and LuxI upstream) whose external concentration increases as a function of increasing cell-population density. Bacteria detect the accumulation of a minimal threshold stimulatory concentration of these autoinducers and regulate the expression of LuxCDABE. Consequently, we removed these regulatory proteins to ensure that cell concentration dependent luminescence through quorum sensing is surpassed and our cultures can fluoresce independently.