Difference between revisions of "Part:BBa K2332017:Design"

 
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===Design Notes===
 
===Design Notes===
 +
We removed the stop codong in GFP and placed the SpyTag sequence
 
To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.
 
To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.
−
 
−
 
−
 
  
 
===Source===
 
===Source===

Revision as of 21:52, 22 October 2017


GFP-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We removed the stop codong in GFP and placed the SpyTag sequence To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.

Source

https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence https://parts.igem.org/Part:BBa_E0040 was for the GFP

References