Difference between revisions of "Part:BBa K2332010:Design"

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===Design Notes===
 
===Design Notes===
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We decided to fuse SpyTag [1] to the N-terminal of a truncated version of Intimin [2] as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface.
+
We decided to fuse SpyTag to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface.
  
 
===Source===
 
===Source===

Revision as of 21:34, 22 October 2017


Intimin'-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to fuse SpyTag to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface.

Source

Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli. Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag was obtained from: BBa_K1159201

References