Difference between revisions of "Part:BBa K2332010:Design"

(→‎Design Notes)
(→‎Source)
Line 11: Line 11:
 
===Source===
 
===Source===
  
−
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences
+
Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli.
−
And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html
+
 
Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001
 
Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001
−
SpyTag: BBa_K1159201
+
SpyTag was obtained from: [https://parts.igem.org/Part:BBa_K1159201 BBa_K1159201]
  
 
===References===
 
===References===

Revision as of 21:33, 22 October 2017


Intimin'-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to fuse SpyTag [1] to the N-terminal of a truncated version of Intimin [2] as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface.

Source

Intimin protein sequence obtained from UniProtKB: [http://www.uniprot.org/uniprot/P43261#sequences P43261], reverse translated and codon optimised for E. coli. Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag was obtained from: BBa_K1159201

References