Difference between revisions of "Part:BBa K2332010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We decided to fuse SpyTag to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins | + | We decided to fuse SpyTag [1] to the N-terminal of a truncated version of Intimin [2] as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface. |
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===Source=== | ===Source=== | ||
Revision as of 21:28, 22 October 2017
Intimin'-SpyTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 801
Illegal NgoMIV site found at 1542 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to fuse SpyTag [1] to the N-terminal of a truncated version of Intimin [2] as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins. We also included a linker to facilitate mobility of SpyTag on the cell surface.
Source
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201