Difference between revisions of "Part:BBa K2332002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We created several constructs comprised of this promoter and reporters. We then have to fuse the EL222 coding gene downstream of each reporter to test blue light induction.
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Designed by Jayaraman P. et al. (2016)
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Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.
  
 
===Source===
 
===Source===

Revision as of 21:06, 22 October 2017


Blue light inducible promoter (Pblind)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed by Jayaraman P. et al. (2016)

Jayaraman P, Devarajan K, Chua T, Zhang H, Gunawan E, Poh C. Blue light-mediated transcriptional activation and repression of gene expression in bacteria. Nucleic Acids Research. 2016;44(14):6994-7005.

Source

Obtained as a synthetic gBlock

References