Difference between revisions of "Part:BBa K2463000:Design"

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====iGEM registry entries regarding parts:====
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====iGEM registry parts:====
 
[https://parts.igem.org/Part:BBa_B0034 BBa_B0034] - Ribosome binding site
 
[https://parts.igem.org/Part:BBa_B0034 BBa_B0034] - Ribosome binding site
 
<br>[https://parts.igem.org/Part:BBa_K2012002 BBa_K2012002] - <b>PLeD gene</b> <i>(synthesises c-di-GMP)</i>
 
<br>[https://parts.igem.org/Part:BBa_K2012002 BBa_K2012002] - <b>PLeD gene</b> <i>(synthesises c-di-GMP)</i>

Revision as of 20:38, 22 October 2017


Biofilm and Motility Regulation Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1806
    Illegal BamHI site found at 32
    Illegal BamHI site found at 1190
    Illegal XhoI site found at 1768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
    Illegal NgoMIV site found at 595
    Illegal AgeI site found at 931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 105


Design Notes

Lacks a degrading tag fused within yhjH gene due to unpredictable interactions.


Source

Uniprot entries for important proteins:

PLeD Source: [http://www.uniprot.org/uniprot/Q9A5I5 uniprot.org/uniprot/Q9A5I5]
yhjH Source: [http://www.uniprot.org/uniprot/P37646 uniprot.org/uniprot/P37646]

iGEM registry parts:

BBa_B0034 - Ribosome binding site
BBa_K2012002 - PLeD gene (synthesises c-di-GMP)
BBa_C0072 - Mnt repressor
BBa_B1006 - Terminator
BBa_R0073 - Mnt promoter
BBa_K861090 - Yhjh (degrades c-di-GMP)
BBa_B1003 - Second Terminator