Difference between revisions of "Part:BBa K2379010"

Revision as of 19:24, 22 October 2017

Adaptor with FourU RNA thermometer

This construct consists of the adaptor(BBa_K2379008) and is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, the RNA Thermometer (BBa_K115002) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by the FourU RNA Thermometer, the circuit will get activated and GFP will be produced only at 37 degree Celsius.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1068

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 <partinfo>BBa_K2379010 short</partinfo>
-This construct consists of the adaptor(BBa_K2379008) and is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, the RNA Thermometer (BBa_K115002) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by the RNA Thermometer, the circuit will get activated and GFP will be produced only at 37 degree Celsius.
+This construct consists of the adaptor(BBa_K2379008) and is under the influence of the pLac promoter(BBa_R0011). In addition to the adaptor, the RNA Thermometer (BBa_K115002) is used to regulate the transcription of the genes downstream. Following the adaptor is the reporter protein GFP (BBa_E0040) and the double terminator(BBa_B0014). Since it is regulated by the FourU RNA Thermometer, the circuit will get activated and GFP will be produced only at 37 degree Celsius.
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