Difference between revisions of "Part:BBa K2203001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | extension PCR to add T7 promoter and terminator and the 6XHis-tag | + | extension PCR to add T7 promoter and terminator and the 6XHis-tag to the gamS coding sequence(BBa_K2203000) |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 18:37, 22 October 2017
T7-GamS-HisTag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 336
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 323
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
extension PCR to add T7 promoter and terminator and the 6XHis-tag to the gamS coding sequence(BBa_K2203000)
Source
pBADmod1-linker2-gamS
References
https://www.addgene.org/45833/