Difference between revisions of "Part:BBa K2203001:Design"

 
(Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
pBADmod1-linker2-gamS (https://www.addgene.org/45833/)
+
pBADmod1-linker2-gamS
  
 
===References===
 
===References===

Revision as of 18:36, 22 October 2017


T7-GamS-HisTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 323
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

extension PCR to add T7 promoter and terminator and the 6XHis-tag


Source

pBADmod1-linker2-gamS

References