Difference between revisions of "Part:BBa K2203000"

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gamS protein

GamS is a protein that protects linear DNA templates from degradation in a cell- free environment. It is used to enhance cell-free protein synthesis by inhibiting exonuclease activity.

This part has a demonstrated positive effect on protein synthesis in a cell-free chassis. GamS can be added to a cell-free reaction system in several ways: By adding it in its synthesized protein form, or by adding simply its coding sequence to the cell-free reaction, or (in the case of a self-made cell lysate) by making the lysate from cells which were transformed with the GamS-plasmid. For the first and last example, see experimental results below.

Sequence and Features