Difference between revisions of "Cell-free chassis/Homemade E. coli S30"
(→Homemade ''E. coli'' S30) |
(→Homemade ''E. coli'' S30) |
||
Line 3: | Line 3: | ||
{| border="0" width="100%" | {| border="0" width="100%" | ||
|- align=center | |- align=center | ||
− | ||<font face=georgia color=#cc0033 size=4>Cell-Free Systems</font> | + | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems <font face=georgia color=#cc0033 size=4>Cell-Free Systems</font>] |
− | ||<font face=georgia color=#000066 size=4>Chassis description</font> | + | ||[https://parts.igem.org/wiki/index.php/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 <font face=georgia color=#000066 size=4>Chassis description</font>] |
||<font face=georgia color=#000066 size=4>Optimum temperature</font> | ||<font face=georgia color=#000066 size=4>Optimum temperature</font> | ||
||<font face=georgia color=#000066 size=4>Peak time</font> | ||<font face=georgia color=#000066 size=4>Peak time</font> |
Revision as of 12:17, 21 October 2007
Homemade E. coli S30
Cell-Free Systems | Chassis description | Optimum temperature | Peak time | Product stability | Expression capacity | Expression lifespan |
Description
The Homemade E. coli S30 extract was prepared using E. coli strain BL21 (DE3) with loss of function mutations in OmpT endoproteinase and lon protease genes. This E. coli strain can express T7 RNA polymerase under the control of LacI, which is inducible by IPTG 1.
Please visit our [http://openwetware.org/wiki/IGEM:IMPERIAL/2007/CFS/Design/Protocols OpenWetWare]for the detailed protocol for preparing Homemade E. coli S30 extract.
References
<biblio>
- 1 pmid=16797767
</biblio>