Difference between revisions of "Part:BBa K2407109:Design"

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===Design Notes===
 
===Design Notes===
 
This sequence is modified for use in yeast as the terminator of HO.
 
This sequence is modified for use in yeast as the terminator of HO.
 +
This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c.
  
 
===Source===
 
===Source===

Revision as of 11:23, 22 October 2017


a terminator used in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast as the terminator of HO. This gene encodes a subunit of the cytochrome bc1 complex, which plays an important role in the mitochondrial respiratory chain by transferring electrons from the Rieske iron-sulfur protein to cytochrome c.

Source

We use PCR to amplify the part from a PRS-415 CAS9 plasmid.

References