Difference between revisions of "Part:BBa K2407107:Design"
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| − | We use PCR to amplify the part from | + | We use PCR to amplify the part from prs416 carotene plasmid. |
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===References=== | ===References=== | ||
Revision as of 10:31, 22 October 2017
HO gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
Illegal BglII site found at 954 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1290
Design Notes
It is a constitutive promoter. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA
Source
We use PCR to amplify the part from prs416 carotene plasmid.