Difference between revisions of "Part:BBa K2407106:Design"
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===Source=== | ===Source=== | ||
| − | We use PCR to amplify the part from a plasmid. | + | We use PCR to amplify the part from a plasmid CAN-A which contains a half part of gene CAN and has TEF1 as its promoter. |
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===References=== | ===References=== | ||
Revision as of 08:52, 22 October 2017
a constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 840
Design Notes
It is a constitutive promoter. It is translation of translational elongation factor EF-1 alpha. When it is the GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides.It also associates with vacuolar Rho1p GTPase. TEF1 may also have a role in tRNA re-export from the nucleus. TEF1 has a paralog, TEF2, that arose from the whole genome duplication.
Source
We use PCR to amplify the part from a plasmid CAN-A which contains a half part of gene CAN and has TEF1 as its promoter.