Difference between revisions of "Part:BBa K2407106:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This sequence is modified for use in yeast or other AT-rich fungi.
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It is a constitutive promoter.
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It is translation of translational elongation factor EF-1 alpha.
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When it is the GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides.It also associates with vacuolar Rho1p GTPase. TEF1 may also have a role in tRNA re-export from the nucleus. TEF1 has a paralog, TEF2, that arose from the whole genome duplication.
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===Source===
 
===Source===

Revision as of 08:48, 22 October 2017


a constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 840


Design Notes

It is a constitutive promoter. It is translation of translational elongation factor EF-1 alpha. When it is the GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides.It also associates with vacuolar Rho1p GTPase. TEF1 may also have a role in tRNA re-export from the nucleus. TEF1 has a paralog, TEF2, that arose from the whole genome duplication.

Source

We use PCR to amplify the part from a plasmid.


References