Difference between revisions of "Part:BBa K2407105:Design"

(→‎Design Notes)
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===References===
 
===References===
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McIsaac, R. Scott & Oakes, Benjamin & Wang, Xin & A Dummit, Krysta & Botstein, David & Noyes, Marcus. (2012). Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast. Nucleic acids research. 41. . 10.1093/nar/gks1313.

Latest revision as of 08:39, 22 October 2017


a transcription factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
    Illegal BglII site found at 737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1494


Design Notes

This sequence is a transcription factor working with Modified-Gal promoter. In the absence of β-estradiol, the Z4EV zinc finger protein is sequestered in the cytoplasm by HSP90. If B-estradiol is added, the Z4EV protein dissociates from the HSP90, revealing a nuclear localization signal (NLS) that translocates the protein to the nucleus and activating the Z4EV promoter. Activation of the Z4EV promoter activates transcription of the vika enzyme which in turn cut the vox site of cu and RFP plasmids.

Source

We obtain it from Gene synthesis company after codon optimization.

References

McIsaac, R. Scott & Oakes, Benjamin & Wang, Xin & A Dummit, Krysta & Botstein, David & Noyes, Marcus. (2012). Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast. Nucleic acids research. 41. . 10.1093/nar/gks1313.