Difference between revisions of "Part:BBa K2302008"

Revision as of 07:51, 22 October 2017

PelB+CALB+His

This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1023
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 25
Illegal NgoMIV site found at 51
Illegal NgoMIV site found at 744
Illegal NgoMIV site found at 854
Illegal AgeI site found at 546
Illegal AgeI site found at 804
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2302008 short</partinfo>
-it contains three enzymes of the serine synthesis pathway. Bacteria harboring this plasmidcan be induced by arabinose to synthesize and secrete serine. A SD sequence (RBS) were added to the front of each coding sequence of serA, serB and serC.
+This plasmid cords for lipase out the membrane,which can hydrolyze fat to fatty acids, it consists four basic parts: promoter T7, RBS, pelB and CALB. pelB is sequence cords for a functional signal peptide, which transport the fused protein CALB out the membrane efficiently, and CALB is a kind of lipase which can hydrolyze fat. By transforming this plasmid, a bacteria can secret lipase out the membrane,hydrolyzing fat in the environment to fatty acids.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===