Difference between revisions of "Part:BBa K2350010"
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| − | To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction which is retrieved from | + | To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction which is retrieved from Synechococcus elongatus PCC 7942 in our experiment. Rrnb is one of terminators available in cyanobacteria PCC 7942. |
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Latest revision as of 07:48, 22 October 2017
PrbcL+MelA+rrnb
To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction which is retrieved from Synechococcus elongatus PCC 7942 in our experiment. Rrnb is one of terminators available in cyanobacteria PCC 7942.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1311
Illegal XhoI site found at 433 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1505
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 589
Illegal BsaI.rc site found at 880