Difference between revisions of "Part:BBa K2350010"

 
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<partinfo>BBa_K2350010 short</partinfo>
 
<partinfo>BBa_K2350010 short</partinfo>
  
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To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction, which is retrieved from pBR322 in our experiment. Rrnb is one of terminators available in cyanobacteria PCC 7942.  
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To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction which is retrieved from pBR322 in our experiment. Rrnb is one of terminators available in cyanobacteria PCC 7942.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:14, 22 October 2017


PrbcL+MelA+rrnb

To produce melanin in microalgae, MelA gene has to be transformed into the cell. PrbcL(BBa_K2350003) is the particular promoter that can overexpress foreign genes in cyanobacteria. The intrinsic promoter of Rubisco large subunit (PrbcL) was chosen as the target for vector construction which is retrieved from pBR322 in our experiment. Rrnb is one of terminators available in cyanobacteria PCC 7942.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1311
    Illegal XhoI site found at 433
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 589
    Illegal BsaI.rc site found at 880