Difference between revisions of "Part:BBa K2450501:Design"

 
 
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===Design Notes===
 
===Design Notes===
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tba
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The TorA leader sequence must be at the N terminal end.
  
 +
The part has been designed so that the sfGFP stays attached to the membrane when the TEV protease cleaves the quencher, and the dark quencher is at the C terminus. This allows the quencher to be removed during PCR to produce a protein without the quencher. This part without the fluorophore can be used to compare the increase in fluorescence against, when the quencher is cleaved off. 
  
  

Latest revision as of 20:04, 21 October 2017


SpyCatcher sfGFP quencher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 397
    Illegal SapI.rc site found at 1291


Design Notes

The TorA leader sequence must be at the N terminal end.

The part has been designed so that the sfGFP stays attached to the membrane when the TEV protease cleaves the quencher, and the dark quencher is at the C terminus. This allows the quencher to be removed during PCR to produce a protein without the quencher. This part without the fluorophore can be used to compare the increase in fluorescence against, when the quencher is cleaved off.


Source

tba

References