Difference between revisions of "Part:BBa K2332040:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Removal of ATG from GFP fusion protein
+
Removal of ATG from GFP for a more efficient expression of this fusion protein
−
 
+
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 15:16, 21 October 2017


Blue light inducible expression of Intimin'-GFP-SpyTag (Pblind Intimin'-GFP-SpyTag)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2844
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2844
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2844
    Illegal NgoMIV site found at 880
    Illegal NgoMIV site found at 1621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2715


Design Notes

Removal of ATG from GFP for a more efficient expression of this fusion protein

Source

Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201 GFPmut 3b: Part:BBa_E0040


References