Difference between revisions of "Part:BBa K2450301:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 
We are using eYFP as it is very bright and detectable in low quantities, and also compatible for detection with CFP and mCherry. The sequence for eYFP comes from AddGene.
 
We are using eYFP as it is very bright and detectable in low quantities, and also compatible for detection with CFP and mCherry. The sequence for eYFP comes from AddGene.
  
−
 
+
We used a medium strength promoter and good RBS, as modelling showed us this would be appropriate for our system.
  
 
===Source===
 
===Source===
  
 
This part uses the Tet operator from BBa_R0040, which is a medium strength promoter.
 
This part uses the Tet operator from BBa_R0040, which is a medium strength promoter.
 +
 
eYFP from AddGene
 
eYFP from AddGene
 +
 +
The RBS is from BBa_B0030.
  
 
===References===
 
===References===

Latest revision as of 15:01, 21 October 2017


pTet promoter coding eYFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We are using eYFP as it is very bright and detectable in low quantities, and also compatible for detection with CFP and mCherry. The sequence for eYFP comes from AddGene.

We used a medium strength promoter and good RBS, as modelling showed us this would be appropriate for our system.

Source

This part uses the Tet operator from BBa_R0040, which is a medium strength promoter.

eYFP from AddGene

The RBS is from BBa_B0030.

References