Difference between revisions of "Part:BBa K2450251:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 
We decided to have a C-terminal CFP tag so it would not interfere with the N-terminal DNA binding domain of the TetR.
 
We decided to have a C-terminal CFP tag so it would not interfere with the N-terminal DNA binding domain of the TetR.
−
 
+
We used CFP so it would be compatible with our other parts, using mCherry and eYFP, and all would be visible in our fluorescent microscope as they have distinct absorption and emission wavelengths.
−
 
+
  
 
===Source===
 
===Source===
  
 
BBa_K106669
 
BBa_K106669
−
TEV cleavage sequence from Merck
+
<p>TEV cleavage sequence from Merck</p>
−
CFP from AddGene
+
<p>CFP from AddGene</p>
  
 
===References===
 
===References===

Latest revision as of 14:44, 21 October 2017


TetR CFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to have a C-terminal CFP tag so it would not interfere with the N-terminal DNA binding domain of the TetR. We used CFP so it would be compatible with our other parts, using mCherry and eYFP, and all would be visible in our fluorescent microscope as they have distinct absorption and emission wavelengths.

Source

BBa_K106669

TEV cleavage sequence from Merck

CFP from AddGene

References