Difference between revisions of "Part:BBa K2450201:Design"
Line 13: | Line 13: | ||
TetR from BBa_K106669 | TetR from BBa_K106669 | ||
− | TEV cleavage sequence from Merck | + | <p>TEV cleavage sequence from Merck</p> |
− | CFP from AddGene | + | <p>CFP from AddGene</p> |
===References=== | ===References=== |
Revision as of 14:40, 21 October 2017
TetR with TEV cleavage site and CFP tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.
Source
TetR from BBa_K106669
TEV cleavage sequence from Merck
CFP from AddGene