Difference between revisions of "Part:BBa K2450201:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
 +
 
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.
 
We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.
−
 
−
 
  
 
===Source===
 
===Source===
  
−
BBa_K106669
+
TetR from BBa_K106669
 
TEV cleavage sequence from Merck
 
TEV cleavage sequence from Merck
 
CFP from AddGene
 
CFP from AddGene
  
 
===References===
 
===References===

Revision as of 14:37, 21 October 2017


TetR with TEV cleavage site and CFP tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to decide on an appropriate location for the TEV cleavage site in TetR. We examined the crystal structure of TetR and identified a linker between the DNA binding and dimerization domains.

Source

TetR from BBa_K106669 TEV cleavage sequence from Merck CFP from AddGene

References