Difference between revisions of "Part:BBa K2450501"

 
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SpyCatcher, sfGFP, TEV cleavable linker, reach quencher
 
SpyCatcher, sfGFP, TEV cleavable linker, reach quencher
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
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This part was designed to be targeted to the outer membrane. It has a TorA leader sequence which targets it to the periplasm through the Tat translocase system. This translocates folded proteins. 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2450501 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2450501 SequenceAndFeatures</partinfo>

Revision as of 14:03, 21 October 2017


SpyCatcher sfGFP quencher

SpyCatcher, sfGFP, TEV cleavable linker, reach quencher


Usage and Biology

This part was designed to be targeted to the outer membrane. It has a TorA leader sequence which targets it to the periplasm through the Tat translocase system. This translocates folded proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 397
    Illegal SapI.rc site found at 1291