Difference between revisions of "Part:BBa K2325003"
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The BBa_K2325003 is a DNA sequence contains N20 associated with sgRNA. The N20-1 is selected from the genome of T7 phage, which is responsible for the synthesis of the DNA packaging protein during phage infection. | The BBa_K2325003 is a DNA sequence contains N20 associated with sgRNA. The N20-1 is selected from the genome of T7 phage, which is responsible for the synthesis of the DNA packaging protein during phage infection. | ||
This part confers resistance against T7 phage upon a strain containing the Cas9 and sgRNA parts. | This part confers resistance against T7 phage upon a strain containing the Cas9 and sgRNA parts. | ||
| − | This part can be considered as an improvement of the (BBa_K1129012), as they contain same N20 sequence but different sgRNA sequence. The way we constructed the part also promoted the function of resistance against T7 phage. | + | This part can be considered as an improvement of the (BBa_K1129012), as they contain same N20 sequence but different sgRNA sequence. We fused crRNA and tracrRNA as a single synthetic guide RNA (sgRNA), which obviated the need for processing the transcribed CRISPR array (pre-crRNA) into individual crRNA components. |
| + | The way we constructed the part also promoted the function of resistance against T7 phage. | ||
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Revision as of 13:50, 21 October 2017
T7 phage N20-sgRNA
The BBa_K2325003 is a DNA sequence contains N20 associated with sgRNA. The N20-1 is selected from the genome of T7 phage, which is responsible for the synthesis of the DNA packaging protein during phage infection. This part confers resistance against T7 phage upon a strain containing the Cas9 and sgRNA parts. This part can be considered as an improvement of the (BBa_K1129012), as they contain same N20 sequence but different sgRNA sequence. We fused crRNA and tracrRNA as a single synthetic guide RNA (sgRNA), which obviated the need for processing the transcribed CRISPR array (pre-crRNA) into individual crRNA components. The way we constructed the part also promoted the function of resistance against T7 phage.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]