Difference between revisions of "Part:BBa K2407100:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
This sequence is modified for use in yeast or other AT-rich fungi.
 
This sequence is modified for use in yeast or other AT-rich fungi.
 +
yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742
  
  

Revision as of 11:54, 21 October 2017


a composite part to control the expression of HO gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal BglII site found at 902
    Illegal BglII site found at 1185
    Illegal BglII site found at 1869
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 866
    Illegal EcoRI site found at 1376
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence is modified for use in yeast or other AT-rich fungi. yangyang miao has used this plasmid to convert the mating type of S.cerevisiae BY4741 to BY4742


Source

We connect the segments(Gal-HO-CYC1) by T4 DNA Ligase.


References