Difference between revisions of "Part:BBa K2332032:Design"

 
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===Design Notes===
 
===Design Notes===
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Amberless E. coli must be used to see the effects of photocageing spyCatcher
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Amberless E. coli must be used to see the effects of photocageing spyCatcher.
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This construct was designed to not contain any other amber stop codon but at position 31 (Lys31X)
  
  

Revision as of 17:43, 20 October 2017


GFP-mutSpyCatcher (Lys31X, for photocaging)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


Design Notes

Amberless E. coli must be used to see the effects of photocageing spyCatcher. This construct was designed to not contain any other amber stop codon but at position 31 (Lys31X)



Source

SpyCatcher: BBa_K1159200 GFPmut 3b: Part:BBa_E0040

References