Difference between revisions of "Part:BBa K2287027"

Revision as of 14:22, 20 October 2017

purF K326Q

It is the coding sequence of the K326Q mutant of glutamine phosphoribosylpyrophosphate amidotransferase that catalyzes the transformation from PRPP to PRA in de novo purine biosynthesis. We introduced a mutation K326Q to the enzyme based on "Identification of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate Amidotransferase by Nucleotides and Relationship to Residues Important for Catalysis"(Gaochao Zhou et al. ,1993) to remit the feedback inhibition by GMP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 876
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 94
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2287027 short</partinfo>
-It is a coding sequence of glutamine phosphoribosylpyrophosphate amidotransferase that catalyzes the transformation from PRPP to PRA in de novo purine biosynthesis. We did a mutation K326Q in this sequence based on "Identification of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate Amidotransferase by Nucleotides and Relationship to Residues Important for Catalysis"(Gaochao Zhou et al. ,1993) to remit the inhibition by GMP.
+It is the coding sequence of the K326Q mutant of glutamine phosphoribosylpyrophosphate amidotransferase that catalyzes the transformation from PRPP to PRA in de novo purine biosynthesis. We introduced a mutation K326Q to the enzyme based on "Identification of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate Amidotransferase by Nucleotides and Relationship to Residues Important for Catalysis"(Gaochao Zhou et al. ,1993) to remit the feedback inhibition by GMP.
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